Day 2 :
Time : 09:00-09:45
Roberto Fernandez-Lafuente has completed his PhD at UAM and postdoctoral studies from UCL-London. He is the leading the “Optimization of biocatalysts and bioprocess” group at ICP-CSIC, a premier Bio-Soft service organization. He has published more than 390 papers in ISI Journal, with an H index of 68 (Scopus) and more than 2200 citations/year, he is coauthor of 20 patents and co-supervisor of 18 doctoral thesis. He is associated editor of Process Biochemistry and has been serving as an editorial board member of more than 20 journals (e.g., Molecules, Enzyme and Microbial Technology, Journal of Biotechnology, Molecular Catalysis, etc.).
Coimmobilization of enzymes is an increasing topic in biocatalysis. It has can save the lag-time that produce the use of enzymes independently immobilized in different particle in casdae reactionss. In some cases, is fully compulsory to prevent the destruction of one of the substrates. However, there some problems directly related to the fact of coimmobilizing two enzymes in one particle. First, it is possible that the best immobilization protocol differs from one enzyme to the other, making necessary to reach compromise solutions. This makes that immobilization cannot be fully utilized to get improved preparations of both enzymes, losing positive impacts in the biocatalyst design. Second, after inactivation of the least stable enzyme, both enzymes need to be discarded, even if the other enzyme remains fully active.
Here we presented a solution to these problems when one enzyme may be stabilized via immobilization, while the other cannot, and the first immobilized/stabilized enzyme is much more stable than the other enzyme. Using as model enzymes a galactosidase and lipases, we will show how we can coat the immobilized/stabilized lipase, then coat the lipase with PEI and finally immobilize the galactosidase. When the galactosidase is inactivated, the lipases remain almost fully active, that way using high buffer concentration the galactosidase may be released. To prevent the PEI release, we have attached it to the enzyme (using glutaraldehyde) and the support (using glyoxyl-octyl). This way, several cycles of lipase reuse after galactosidase inactivation could be performed.
University of Medicine and Pharmacy-Iuliu Hatieganu, Romania
Time : 09:45-10:30
Lia Monica Junie is an MD, Ph D, Professor, Head Microbiology Department, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj Napoca, Romania. She is a Coordinator of resident doctor’s in the Laboratory Medicine specialty and Leadership PhD doctor’s thesis in Medicine field. She is a Board Member of European professional Societies ESCMID (ESGCP Study Groups), Society of Chemotherapy, Scientia Parasitologica ProVita and is a Reviewer of international reviews, Member of International organizations, Director/Coordinator in research projects. She has more than 63 papers published in full in international journals and is an editorial board member of national reviews. She is an Organizer/President, Keynote, Invited Speaker and Chair of International and National Congresses.
Hydatidosis or cystic echinococcosis is an infestation caused by the larval form of E. granulosus species. It may develop asymptomatically or as a severe disease, often fatal, in humans. From all the patients 81% had positive, and 19% negative serology. The hydatid cyst is present in adults (46.5%) and children (53.5%). In adults hydatid cyst prevails in young adults (47%), has an incidence of 34% in adults of 40-60 years of age, 12.9% in those of 60-70 years of age and a low incidence in old people (6.5%). Hydatid cyst is more common in females (53%) than in males (47%). Is present with a percentage of 39.3 of all operated hydatid cysts in Cluj and the rest of the cases come from other areas of the county. Although the total incidence of hydatid cyst in rural communities is only slightly increased (57.9%) than in urban areas (42.1%), in some counties the incidence in village areas is significantly higher than in urban areas. Hepatic (71.5%) and pulmonary (15.5%) locations are frequent, leading by their chronic evolution to pseudotumoral signs, severe complications (1.5%), reserved prognosis (9.5%), especially in adults (14%). Hydatidosis occur both in rural and urban communities, in both genders and all age groups. The modern management of hydatidosis needs the contribution of all diagnosis and therapeutic procedures. There is an urge for the early diagnosis of the infected patients, by the development of the screening methods and a close collaboration among general practitioners, clinicians, laboratory doctors and statisticians in order to correctly diagnose of hydatidosis.